RNA

Part:BBa_K5252007:Design

Designed by: Samuel Williams   Group: iGEM24_EPFL   (2024-09-28)


RDX Riboswitch (clone 4)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 57
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 57
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 57
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 57
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Clone 4 was selected over clone 11 since it has a greater fold change in fluorescence response when coupled to DsRed Express2 when induced with 44 mM RDX. It is also less leaky (less baseline activity in absence of RDX). [1]

References

[1] Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.

https://pubmed.ncbi.nlm.nih.gov/30700648/


Source

From Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.

https://pubmed.ncbi.nlm.nih.gov/30700648/

References

Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.

https://pubmed.ncbi.nlm.nih.gov/30700648/