Part:BBa_K5252007:Design
RDX Riboswitch (clone 4)
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 57
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 57
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 57
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 57
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Clone 4 was selected over clone 11 since it has a greater fold change in fluorescence response when coupled to DsRed Express2 when induced with 44 mM RDX. It is also less leaky (less baseline activity in absence of RDX). [1]
References
[1] Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.
https://pubmed.ncbi.nlm.nih.gov/30700648/
Source
From Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.
https://pubmed.ncbi.nlm.nih.gov/30700648/
References
Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.